《植物生理学报》 2014, 50(9): 1413-1422

低温胁迫对不同百合主要生理指标的影响

王玲丽^2,*, 贾文杰^1,*, 马璐琳¹, 崔光芬¹, 吴丽芳¹, 王祥宁¹, 段青¹, 蒋亚莲¹, 王继华^1,**

¹云南省农业科学院花卉研究所, 云南省花卉育种重点实验室, 昆明650205; ²华中农业大学园艺林学院, 武汉430070

通信作者：王玲丽；E-mail: 1169889638@qq.com；Tel: 138888-22307

摘　要：

本文以12个市场流行的商业品种与2个野生种百合作为实验材料, 研究其幼苗在低温胁迫下的生理机制。通过方差分析与隶属函数分析对低温胁迫后的幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、脯氨酸(Pro)、相对电导率(REC)、丙二醛(MDA)的变化规律进行研究, 并对14个品种(种)及百合各系列抗寒能力进行综合评价。分析结果表明, 植物的抗寒机制是一个复杂的生理生化过程, 其抗寒能力的强弱是由多种复杂的因素控制的结果, 而非单纯的单一因素所决定。14个百合品种(种)的抗寒能力大小依次为: ‘佳娜’＞‘洛宾娜’＞‘西伯利亚’＞‘黄丝带’＞‘蒙特马什’＞兰州百合＞岷江百合＞‘白狐狸’＞‘雪皇后’＞‘王室落日’＞‘王室之歌’＞‘发光’＞‘里昂’＞‘布鲁勒’。百合品种(种)间抗寒性差异较大, 而系列内差异较小。

关键词：百合; 抗寒性; 抗氧化酶; 丙二醛; 相对电导率; 脯氨酸

收稿：2014-04-14   修定：2014-07-21

资助：国家科技部“863”项目(2011AA100208)、云南省科技计划项目(2012BB011)、国家观赏园艺工程技术研究中心(2012FU125X10)和云南省农业科学院创新团队(YAAS-2014TD002)。

Influences of Low Temperature Stress on the Main Physiological Indexes of Different Lilium

WANG Ling-Li^2,*, JIA Wen-Jie^1,*, MA Lu-Lin¹, CUI Guang-Fen¹, WU Li-Fang¹, WANG Xiang-Ning¹, DUAN Qing¹, JIANG Ya-Lian¹, WANG Ji-Hua^1,**

¹Yunnan Key Lab of Flower Breeding, Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, China; ²College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China

Corresponding author: WANG Ling-Li; E-mail: 1169889638@qq.com; Tel: 138888-22307

Abstract:

We studied on the physiological mechanism of different Lilium seedlings under low temperature stress, used 12 market popular commercial varieties and 2 wild species lilies as experimental materials. By variance analysis and subordinate function analysis of variations in SOD activities, POD activities, CAT activities, proline contents, relative electric conductivities, MDA contents of lily seedlings under low temperature stress, a comprehensive evaluation of cold resistance had been made. The results showed that cold resistance mechanism of plants was a complex physiological and biochemical processes, the strength of their cold resistance was controlled by complex factors, rather than a single factor. The strength of cold resistance in the order was: ‘Janna’>‘Robinna’>‘Siberia’>‘Yellow Ween’>‘Montde Marsan’>L. davidii var unicolor>L. regale>‘White Fox’>‘Snow Queen’>‘Royal Sunset’>‘Royal Song’>‘Ceb Glow’>‘Lyon’>‘Brunello’. There were big differences of cold resistance between different varieties (species) of lily. However, the differences of cold resistance between different series were small.

Key words: Lilium spp.; cold resistance; antioxidant enzyme; MDA; relative electric conductivity; proline